Lavigne Laboratories, LLC
 
 
Frequently Asked Questions
Syringeless F.A.Q.

General
1.1 What’s the price of a Syringeless Injector?
1.2 Do I need to purchase any special equipment for the Syringeless Injector?
1.3 Do you have a Users Manual that I can download?
1.4 Can I get my samples analyzed by Syringeless Injection?

Installation
2.1 Do I need to make and any permanent changes to my Gas Chromatograph to install the Syringeless Injector?
2.2 Is it complicated to install or operate?
2.3 About how long will it take to install the Syringeless Injector?

Operation
3.1 How do the volatiles transfer from my sample into the capillary GC column?
3.2 What causes the Sample Vial to move into the Injection Port?
3.3 What causes the Sample Vial to be expelled from within the Injection Port?
3.4 How do I control the amount of time the sample spends within the injection port?
3.5 How many times is the total volume of the Sample Vial flushed with carrier gas during the transfer of volatiles into the GC column?
3.6 At what temperatures can I operate the Syringeless Injector?
3.7 Does the Syringeless Injector operate with packed GC columns?
3.8 Once my sample has returned to the sample loading area, can I move the Sample Loading Valve from RUN to LOAD position?
3.9 Do I need to use cryogenics (LN2 or CO2) to focus the sample volatiles within the capillary GC column?
3.10 What is the shortest duration the Sample Vial can spend within the Injection port transferring volatiles into the column?
3.11 Is there a problem with memory effects between runs?


General
1.1 What’s the price of a Syringeless Injector?

A.Syringeless Injector Kit $8,600.00 U.S.

1.2 Do I need to purchase any special equipment for the Syringeless Injector?

A. The Syringeless Injector comes with everything you need to get started. A milligram balance and Bunsen burner would be helpful.

1.3 Do you have a Users Manual that I can download?

A. Yes, click here Syringeless Users Manual ~ 7.0Mbytes

1.4 Can I get my samples analyzed by Syringeless Injection?

A Yes, we have a number of options starting from, no charge to comprehensive analysis at competitive rates. Contact information

Installation
2.1 Do I need to make and any permanent changes to my Gas Chromatograph to install the Syringeless Injector?

A. No, the Syringeless Injector simply replaces your injection port liner.

2.2 Is it complicated to install or operate?

A Installation is similar to changing your injection port liner. Operation involves simply rotating the Load/Run valve or either Load to load a sample or Run to inject or remove the sample.

2.3 About how long will it take to install the Syringeless Injector?

A. With a little experience, you should be able to switch from syringe injection to Syringeless in about 5 minutes.

Operation
3.1 How do the volatiles transfer from my sample into the capillary GC column?

A. Your sample, which is contained within a Sample Vial, is moved into the injection port and surrounds the capillary GC column. Volatiles from your sample are swept with carrier gas into the column at the same pressure and flow rate as the capillary GC column. There is no need to expose your sample to elevated pressures in excess of your injection port pressure in order for the volatiles to transfer into the GC column.

3.2What causes the Sample Vial to move into the Injection Port?

A. Gravity, when the Split Vent carrier gas supporting the sample vial is removed the sample tube falls into the Injection Port.

3.3 What causes the Sample Vial to be expelled from within the Injection Port?

A. Split Vent carrier gas, as the carrier gas enters the bottom of the Injection Port it travels up and out through the Split Vent carrying the Sample Vial with it.

3.4 How do I control the amount of time the sample spends within the injection port transferring volatiles into the capillary GC column?

A. With the injection ports Split / Splitless valve controller. When operating in the Split Mode the sample vial is suspended in a flow of carrier gas at the top of the Syringeless Injectors sample loading area. Switching to Splitless Mode removes the supporting carrier and the sample tube falls into the injection port. The duration of time you choose stay in the Splitless Mode determines the extraction time. Switching back to Split Mode expels the Sample Vial.

3.5 How many times is the total volume of the Sample Vial flushed with carrier gas during the transfer of volatiles into the GC column?

A. It depends on your column flow rate and extraction time. Example: Column flow rate (1.5ml./min) x extraction time (2.0 min) = 3 ml. Divide by Sample Vial volume of 0.180 ml. This results in a sample vial being flushed 16.6 times during a 2 min extraction.

3.6 At what temperatures can I operate the Syringeless Injector?

A. The full temperature range of your Gas Chromatograph Injection Port, ambient to 400C

3.7 Does the Syringeless Injector operate with packed GC columns?

A. No, the Syringeless Injector has been designed for maximum transfer efficiency of sample volatiles directly into capillary GC columns. It is not a device that has been modified to operate with capillary GC columns from the older packed column technology.

3.8 Once my sample has returned to the sample loading area, can I move the Sample Loading Valve from RUN to LOAD position?

A. Moving the Sample Loading Valve from the RUN to LOAD position during the analysis will have no effect on the chromatography.

3.9 Do I need to use cryogenics (LN2 or CO2) to focus the sample volatiles within the capillary GC column?

A. No, it depends on the type of column phase, phase thickness, initial column temperature and boiling point of the material being collecting.

3.10 What is the shortest duration the Sample Vial can spend within the Injection port transferring volatiles into the column?

A. About 1 second, you will need to add an additional 1 to 2 seconds, to the total injection time, to account for the time needed for the Sample Vial to fall into the Injection port.

3.11 Is there a problem with memory effects between runs?

A. No, a new disposable Sample Vial, which is acting as an Injection Port Liner, is replaced for each injection.

If you have any additional questions please e-mail them to questions@syringeless.com